Hyperhomocysteinemia and cerebrovascular diseases in persons of pre-advance age in districts affected by the accident at the Chernobyl nuclear power plant

  • Yu. I. Bandazhevsky Ecology and Health Coordination and Analytical Centre
  • N. F. Dubova Shupyk National Medical Academy of Postgraduate Education
  • L. A. Ustinova Ukrainian Military Medical Academy https://orcid.org/0000-0002-6582-7231
Keywords: Hyperhomocysteinemia, folate cycle, risk alleles, cerebrovascular diseases, adolescents, radiation contaminated areas.

Abstract

Introduction. During the implementation of European Union projects in Ukraine «Health and environmental programs associated with the Chernobyl exclusion zone. Preparation, training and coordination of health projects» in 2015 hyperhomocysteinemia was observed in a group of adolescents living in districts bordering the Chernobyl exclusion zone in 75.3 % of cases (among males in 86.5 % of cases). An association between hyperhomocysteinemia and pathological processes in the central nervous system has been established. It is important to determine the causes of this phenomenon, including in pre-conscription persons, which limits their suitability for military service.

The purpose of the study was to find out the reasons for the increase in homocysteine levels in male adolescents living in areas contaminated with radioactive elements as a result of the accident at the Chernobyl nuclear power plant.

Materials and methods. In the course of the research, 228 males aged 8-17 years permanently residing in rural settlements of Ivankovsky and Polessky districts of Kyiv region were examined. The following methods were used: bibliosemantic, analytical, molecular genetic diagnostics, statistical.

Results. The examined subjects showed genetic changes affecting the activity of the main enzyme of the folate cycle (FC) - methylenetetrahydrofolate reductase. In particular, the T risk allele of the MTHFR:677 genetic polymorphism was identified in 48.7 % of cases, its homozygous T/T MTHFR:677 variant was found in 7.5 % of cases, and compound heterozygosity for CT677/AC1298 alleles of the MTHFR gene was recorded in 18.9 %. These genetic changes in the FC predispose to impaired functioning of the FC and an increase in the level of homocysteine in the blood, which is the basis for the development of pathological processes affecting vital organs and systems. Abnormal homocysteine metabolism can also be associated with compound heterozygosity of CT MTHFR:677/ AG MTRR:66, AG MTR:2756/AG MTRR:66, AG MTR:2756/CT MTHFR:677 polymorphisms recorded respectively in 21.9 %, 18.0 %, 14.9 % of cases.

Conclusions. The factor that provokes the phenotypic realization of the genetic defects of the FC in the form of hyperhomocysteinemia can be both radioactive elements and other causes of stressful situations. Given that mutations in the FC genes lead to a state of hyperhomocysteinemia, is a risk factor for inappropriate behavior of young people in stressful situations, which limits their suitability for military service, it is advisable to take into account the genetic system of the folate cycle and homocysteine levels in conscripts. their health when called up for military service.

Author Biographies

Yu. I. Bandazhevsky, Ecology and Health Coordination and Analytical Centre

MD, professor, Head of Ecology and Health Coordination and Analytical Centre

N. F. Dubova, Shupyk National Medical Academy of Postgraduate Education

PhD (Med), Associate Professor, Associate Professor of Department of Public Health

L. A. Ustinova, Ukrainian Military Medical Academy

Col MS, MD DSc, Professor, Head of Department of Military Toxicology, Radiology and Medical Protection

References

Bandazhevsky, Yu.I., Dubova, N.F. (2017). State of folate metabolism and health of children living in areas contaminated with radioactive elements as a result of the Chernobyl nuclear power plant accident. Scientific and practical collection «Chernobyl: ecology and health». Ivankov: PI Coordination and Analytical Center «Ecology and health». Dnipro: Serednyak Т.К., Issue 6. P. 16-26.

Skovierova, H., Vidomanova, E., Mahmood, S., Sopkova, J., Drgova, A., Cervenova, T., Halasova, E. and Lehotsky, J. (2016). The molecular and cellular Effect of homocysteine metabolism imbalance on human health. Int. J. Mol. Sci. V. 17. № 10. P.1710-1733. https://doi.org/10.3390/ijms17101733

Evtushenko S.K., Filimonov D.A. (2013). Rol gomotsisteina v razvitii ishemicheskih insultov u lits molodogo vozrasta (obzor literaturyi i lichnyie nablyudeniya). International Neurological Journal. V. 7 (61). 19-30. [In Russian]

Zieminska, E., Stafiej, A., Lazarewicz, J.W. (2003). Role of group I metabotropic glutamate receptors and NMDA receptors in homocysteine-evoked acute neurodegeneration of cultured cerebellar granule neurons. Neurochem. Int. 43(4-5). 481-492. https://doi.org/10.1016/s0197-0186(03)00038-x

Maron, B., Loscalzo, J. (2009). The Treatment of Hyperhomocysteinemia. Annu Rev Med. V. 60. P. 39–54. https://doi.org/10.1146/annurev.med.60.041807.123308

Keshteli, A, Baracos, V, Madsen, K. (2015). Hyperhomocysteinemia as a potential contributor of colorectal cancer development in inflammatory bowel diseases: A review. World J Gastroenterol. Jan 28; V. 21(4). 1081–1090. https://doi.org/10.3748/wjg.v21.i4.1081

Polushin, A.Yu., Odinak, M.M., Yanishevsky, S.N., Golokhvastov, S.Yu., Tsygan, N.V. (2013). Gipergomotsisteinemiya – prediktor tyazhesti insulta na fone obshirnosti povrezhdeniya mozgovogo veschestva. Bulletin of the Russian Military Medical Academy. V. 4 (44). 89-94. [In Russian]

Myasoedov, V.V., Zhukov, V.I. (2004). Retseptornyie mehanizmyi paroksizmalnyih sostoyaniy (obzor). Experimental i clinical medicine. 2004. V. 1. 5-12. [In Russian]

Boldyrev, A.A. (2009). Molekulyarnyie mehanizmyi toksichnosti gomotsisteina. Biochemistry. V. 74 (6). 725-736. [In Russian]

Varshney, K.K., Gupta, J.K. and Mujwar, S. (2019). Homocysteine induced neurological dysfunctions: A link to neurodegenerative disorders. International Journal of Medical Research & Health Sciences. V. 8. № 4. 135-146. https://www.ijmrhs.com/medical-research/homocysteine-induced-neurological-dysfunctions-a-link-to-neurodegenerative-disorders.pdf

Bandazhevsky, Yu.I., Dubova, N.F. (2017). The role of genome of folate metabolism in the occurrence of hyperhomocysteinemia in children from the areas affected by the Chernobyl nuclear power plant accident. Scientific and practical collection «Chernobyl: ecology and health». Ivankov: PI Coordination and Analytical Center «Ecology and health», - Dnipro: Serednyak Т.К.. V. 6. 27-35.

Bandazhevsky, Yu.I., Dubovaya, N.F. (2019). The state of folate metabolism and its link with thyroid system in children after forest fires in the Chornobyl exclusion zone. Environment&Health. V. 2 (91). 10-16.

Zahalnodozymetrychna pasportyzatsiia ta rezultaty LVL-monitorynhu v naselenykh punktakh Ukrainy, yaki zaznaly radioaktyvnoho zabrudnennia pislia Chornobylskoi katastrofy. Dani za 2011 r. COLLECTION 14. K .: Ministry of Health of Ukraine, National Academy of Medical Sciences of Ukraine, Ministry of Emergencies of Ukraine, DAZV, State Institution "NSCRM NAMS of Ukraine", ND IRZ ATN of Ukraine, 2012. 99 p. [In Ukranian]

Zhilyaeva, T.V., Sergeeva, A.V., Blagonravova, A.S., Kasimova, L.N. (2016). Psihopatologicheskaya harakteristika i osobennosti sotsialnogo funktsionirovaniya bolnyih shizofreniey s nositelstvom T-allelya v polimorfnom lokuse gena obmena folatov MTHFR677C> T. Journal of Neurology and Psychiatry. V. 11. 5-11. [In Russian]

Pankova, E.E., Zinchenko, L.V., Matulevich, S.A., Golubtsov, V.I. (2009). Polimorfizm S677T gena MTHFR kak faktor riska vrozhdennoy patologii u potomstva. Kuban Scientific Medical Bulletin. V. 6 (111). 144-147. [In Russian]

Karpenko, M.A., Shatskaya, E.G., Solntsev, V.N. et al. (2008). Ostryie tserebrovaskulyarnyie katastrofyi u bolnyih arterialnoy gipertenziey: molekulyarno-geneticheskie aspektyi. Rational pharmacotherapy in cardiology. V. 1. 33-38. [In Russian]

Zhang, X., Liu, T., Li, Y., Li, S. (2015). Association between MTHFR 677C/T and 1298A/C gene polymorphisms and breast cancer risk. Genet. Mol. Res. V. 14. № 4. 16425-16430. https://doi.org/10.4238/2015.December.9.12

Grechanina, E.Ya., Malaton, R., Grechanina, Yu.B. and other (2008). Nasledstvennyie narusheniya obmena serosoderzhaschih aminokislot. Russian Bulletin of Perinatology and Pediatrics. V. 6. 57-65. [In Russian]

Vayner, A.S., Kudryavtseva, E.A., Zhechev, D.A. et al. (2011). Issledovanie assotsiatsii polimorfizma genov folatnogo obmena s riskom vrozhdennyih anomaliy razvitiya. Mother and child in Kuzbass. V. 3 (46). 10-16. [In Russian]

Bandazhevsky, Yu.I., Dubova, N.F. (2018). Genome of folate metabolism and folic acid deficiency in children living in areas affected by the Chernobyl nuclear power plant accident. Collected scientific works of employees of NMAPE named after P.L. Shupik. V. 29. 304-311.

Bandazheuski, Yu., Dubovaya, N. (2018). MTHFR: 677ТТ genotype and the level of vitamin B9 in children, who live in the areas affected by the Chernobyl nuclear power plant accident. Pediatrics. Eastern Europe. V. 6, № 2. 249-255.

Bandazhevsky, Yu.I., Dubovaya, N.F., Kadun, O.N. (2019). Age-specific features of 137Cs concentrations in children from Ivankovsky and Polessky districts in Kiev region, Ukraine, 30 years after the Chernobyl nuclear power plant accident. Collection of Scientific and Practical Articles «Chernobyl: ecology and health»: Under general editorship of Prof. Yu.I. Bandazhevsky. Ivankov: PI Coordination and Analytical Center «Ecology and health», - Dnipro: Serednyak Т.К. Issue 9. 4 -10.

Bandazhevsky, Yu.I., Dubovaya, N.F. (2019). Physical growth of children in the presence of 137Cs incorporation 30 years after the Chernobyl nuclear power plant accident. Collection of Scientific and Practical Articles «Chernobyl: ecology and health»: Under general editorship of Prof. Yu.I. Bandazhevsky. Ivankov: PI Coordination and Analytical Center «Ecology and health», - Dnipro: Serednyak Т.К. Issue 9. 29-41.

Bandazhevskiy, Yu.I., Dubova, N.F. (2020). Pozhezhi lisu v Chornobylskii zoni vidchuzhennia ta zdorov´ia ditei. Ivankiv: PU Coordination Analytical Center “Ecology and I am healthy, - Dnipro: Serednyak T.K. 42 p. [In Ukranian]

Bandazhevsky, Yu.I., Dubova, N.F. (2017). Dynamics of hyperhomocysteinemia in children living in areas contaminated with radionuclides as a result of the Chernobyl nuclear power plant accident. Scientific and practical collection «Chernobyl: ecology and health». Ivankov: PI Coordination and Analytical Center «Ecology and health», Dnipro: Serednyak Т.К. Issue 6. 36-40.

Sheybak, V.M., Lelevich, V.V., Bandazhevsky, Yu.I. (1995). Amino acides of blood plasma and tissues of animals after of cesium and strontium radionuclides. Clinical and experimental aspects of the effect of incorporated radionuclides upon the organism / Ed. by Yu.I. Bandazhevsky, V.V. Lelevic. Gomel. 83-106.

Published
2020-12-31
How to Cite
Bandazhevsky, Y. I., Dubova, N. F., & Ustinova, L. A. (2020). Hyperhomocysteinemia and cerebrovascular diseases in persons of pre-advance age in districts affected by the accident at the Chernobyl nuclear power plant. Ukrainian Journal of Military Medicine, 1(4), 20-28. https://doi.org/10.46847/ujmm.2020.4(1)-020
Section
Military preventive medicine