The study of texture and pharmacological properties of a new rectal medicine

Keywords: textural indicators, pharmacological activity, thick extract of carrot roots, rutin, cream, mucoadhesion.

Abstract

Purpose. Study of textural and pharmacological properties of a new cream with thick carrot extract intended for the treatment of hemorrhoids.

Materials and methods. The object of the research is samples of a new cream with different concentrations of two mucoadhesive agents - sodium alginate and Carbopol® 980 and a comparison drug - "Procto-Glivenol Cream". The subject of the research is textural and pharmacological properties of samples of a new rectal cream. Research methods: pharmacological, structural-mechanical, physical.

Results. The dynamic viscosity and adhesion time of the studied samples were determined. The correlation of dynamic viscosity indicators and adhesion time is shown depending on the concentration of sodium alginate and Carbopol® 980. It was established that the samples with the addition of Carbopol® 980 have indicators at the level of the comparison drug. It was determined that the samples of the drug based on Carbopol have a degree of spreading at the level of the comparison drug, which will ensure a more uniform distribution on the surface of the mucous membrane during rectal administration.

Conclusions. Textural studies were conducted to determine the type and content of the mucoadhesive agent in the composition of a soft rectal medicinal product with carrot extract. The feasibility of using Carbopol® 980 as a mucoadhesive agent at a concentration of 0.4% was determined based on the results of determining dynamic viscosity, adhesion time, and linear dimensions of spreading under load. According to the results of pharmacological studies, the presence of a dose-dependent effect of the cream with Carrot extract and Rutin was established, which was able to reduce the permeability of blood vessels in the rectoanal area of rats with experimental hemorrhoids. The drug in a dose of 300 mg/kg caused a decrease in the permeability of vessels of the rectoanal zone by 54.8%.

Author Biographies

О. А. Ruban, Drugs National University of Pharmacy

Doctor of pharmaceutical sciences, professor  Head Department of Industrial Technology of Drugs National University of Pharmacy.

Mohammad Al Sayasneh, Drugs National University of Pharmacy

graduate student, Department of Industrial Technology of Drugs National University of Pharmacy.

D. V. Lytkin, Drugs National University of Pharmacy

Candidate (PhD) of Biology, Educational and Scientific Institute of Applied Pharmacy, National University of Pharmacy.

I. V. Kovalevska, Drugs National University of Pharmacy

Doctor of pharmaceutical sciences, Professor, Department of Industrial Technology of Drugs, National University of Pharmacy. 

References

Al Sayasneh Mohammad, Ruban O. A., Kovalevska I. V., Ievtushenko O. M. Аnalysis of the market of medicinal products for the conservative treatment of hemorrhoids in order to determine the marketing opportunities for a domestic manufacturer. Social Pharmacy in Health Care. Vol. 8 No. 4 (2022), 46-58. DOI: https://doi.org/10.24959/sphhcj.22.274http://sphhcj.nuph.edu.ua/article/view/273078

Borko, Y. A., Kovalevska, I. V., Ruban, O. A., & Kutova, O. V. (2022). Development and optimization of quantitative composition of rectal suppositories with diosmin and hesperidin by the method of mathematical planning of the experiment. Farmatsevtychnyi Zhurnal, (1), 74-85. https://doi.org/10.32352/0367-3057.1.22.07

Margetis N. (2019). Pathophysiology of internal hemorrhoids. Annals of gastroenterology, 32(3), 264–272. https://doi.org/10.20524/aog.2019.0355

Sun, Z., & Migaly, J. (2016). Review of Hemorrhoid Disease: Presentation and Management. Clinics in colon and rectal surgery, 29(1), 22–29. https://doi.org/10.1055/s-0035-1568144

Kibret, A. A., Oumer, M., & Moges, A. M. (2021). Prevalence and associated factors of hemorrhoids among adult patients visiting the surgical outpatient department in the University of Gondar Comprehensive Specialized Hospital, Northwest Ethiopia. PloS one, 16(4), e0249736. https://doi.org/10.1371/journal.pone.0249736

Sheikh, P., Régnier, C., Goron, F., & Salmat, G. (2020). The prevalence, characteristics and treatment of hemorrhoidal disease: results of an international web-based survey. Journal of comparative effectiveness research, 9(17), 1219–1232. https://doi.org/10.2217/cer-2020-0159

Lohsiriwat, V. (2012). Hemorrhoids: from basic pathophysiology to clinical management. World journal of gastroenterology, 18(17), 2009–2017. https://doi.org/10.3748/wjg.v18.i17.2009

Hong, Y.S., Jung, K.U., Rampal, S. et al. Risk factors for hemorrhoidal disease among healthy young and middle-aged Korean adults. Sci Rep 12, 129 (2022). https://doi.org/10.1038/s41598-021-03838-z

De Marco, S., & Tiso, D. (2021). Lifestyle and Risk Factors in Hemorrhoidal Disease. Frontiers in surgery, 8, 729166. https://doi.org/10.3389/fsurg.2021.729166

Sun, Z., & Migaly, J. (2016). Review of Hemorrhoid Disease: Presentation and Management. Clinics in colon and rectal surgery, 29(1), 22–29. https://doi.org/10.1055/s-0035-1568144

Stadnytska, N. E., Pavlyuk, I. V., Dumych, I. I., Blonsky, O. V. (2014). Prospective study of the use of wild carrot fruits as a source of new complexes of biologically active substance. Bulletin of the National University "Lviv Polytechnic". Chemistry, technology of substances and their application. 787. 243-248.

Kyslychenko, O. A., Protska, V. V., Zhuravel, I. O., Hutsol, V. V. (2018). The study of Daucus carota subsp. sativus fruits fatty acid composition of «Olenka», «Kharkivska Nantska» and «Yaskrava» varieties. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 9 (6), 307-312. doi: 10.2478/afpuc-2022-0002

Borko, Y., Inna, K., Grudko, V., Kononenko, N., & Velya, M. (2022). Comprehensive study for the development of rectal suppositories with diosmin and hesperidin. ScienceRise: Pharmaceutical Science, 1(35), 14–21. https://doi.org/10.15587/2519-4852.2022.253518

Bioadhesive Polymers for Drug Delivery Eneko Larrañeta, Ryan F. Donnelly Book Editor(s):Carmen Scholz First published: 30 June 2017 https://doi.org/10.1002/9781118967904.ch18

Christina Woertz, Maren Preis, Jörg Breitkreutz, Peter Kleinebudde (2013). Assessment of test methods evaluating mucoadhesive polymers and dosage forms: An overview, European Journal of Pharmaceutics and Biopharmaceutics, 85 (3)б Part B, 843-853.

Ivaniuk, O., Yarnykh, T., & Kovalevska, I. (2019). Determination of the bioadhesion indicators of vaginal gel with resveratrol and hyaluronic acid. Eureka: Health Sciences, (2), 33-39. https://doi.org/10.21303/2504-5679.2019.008800

Kozhem'yakin, Yu.M., Khromov, O.S., Filonenko, M.A., Saifetdinov, G.A.. (2002). Cientific and practical recommendations for keeping laboratory animals and working with them. K: Avicenna, 156

Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. OJEU2010; L276: 33-79.

Azeemuddin, M, Viswanatha, G.L., Rafiq, M., et al. (2014). An improved experimental model of hemorrhoids in rats: evaluation of antihemorrhoidal activity of an herbal formulation. ISRN Pharmacol.530931.. doi:10.1155/2014/530931

Agnieszka Krzyzanowska, Yasmina Martin, Carlos Avendaño et al. (2010). Evaluation of Evans Blue extravasation as a measure of peripheral inflammation, 15 December 2010, PROTOCOL (Version 1) available at Protocol Exchange [https://doi.org/10.1038/protex.2010.209]

Indrayan, A., Malhotra, K. R. (2018). Medical biostatistics. 4th ed. Boca Raton : CRC Press, 685 p.

AAT Bioquest, Inc. (2022, November 17). Quest Graph™ EC50 Calculator. AAT Bioquest. https://www.aatbio.com/tools/ec50-calculator.

Haas, J, Manro, J, Shannon, H, et al. In Vivo Assay Guidelines. 2012 May 1 [Updated 2012 Oct 1]. In: Markossian, S, Grossman, A, Brimacombe, K, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; Available from: https://www.ncbi.nlm.nih.gov/sites/books/NBK92013/Shakir

Mansuri, Prashant Kesharwani, Keerti Jain, Rakesh K. Tekade, Jain, N.K. (2016). Mucoadhesion: A promising approach in drug delivery system, Reactive and Functional Polymers, 100, 151-172.

Published
2023-03-31
How to Cite
RubanО. А., Al Sayasneh, M., Lytkin, D. V., & Kovalevska, I. V. (2023). The study of texture and pharmacological properties of a new rectal medicine. Ukrainian Journal of Military Medicine, 4(1), 174-181. https://doi.org/10.46847/ujmm.2023.1(4)-174