THE INFLUENCE OF COMBINED ABDOMINAL AND THORACIC TRAUMA ON THE ABSORBINGEXCRETING AND GLYCOGEN-SYNTHESIZING FUNCTIONS OF THE LIVER IN THE PERIOD OF EARLY MANIFESTATIONS OF TRAUMATIC DISEASE AND THEIR CORRECTION WITH THIOTRIAZOLINE

  • А.А. Goodyma
  • O.A. Zachepa
  • Yu.I. Sushko
Keywords: combined trauma, thorax, abdomen, bromsulfalein testing, glycogen, thiotriazoline

Abstract

Introduction. Multiorgan insufficiency is considered to be a serious complication of traumatic disease and belongs to the main causes of organism death in the period of its early and late manifestations. A key place among its causes belongs to the combined thoracoabdominal trauma, which occupies the third place in the structure of combat traumas, second to the injuries of the limbs and the head. There are no data on the efficacy of thiotriazoline under these conditions, which is characterized by an evident hepatoprotective effect.
Objective of the study: to find out peculiarities of the liver dysfunction formation in the conditions of combined abdominal and thoracic trauma of rats in the early period of traumatic disease and to evaluate the effectiveness of its correction with thiotriazoline.
Materials and methods. The experiments were conducted on 86 non-linear white male rats weighing 200-220 g. All animals were divided into five groups: a control and four experimental ones. The injuries were carried out under thiopental sodium anesthesia. The control group included intact animals, which were only injected into anesthesia. In the first experimental group animals were with simulated thorax trauma. In the second experimental group blunt abdominal trauma was simulated. In the third experimental group, these injuries were combined. In the fourth experimental group of animals with combined trauma, thiotriazoline was administered intraperitoneally at a dose of 9.1 mg per kg-1. In 1, 3, and 7 days after injuries under conditions of thiopental sodium anesthesia, the common bileduct was catheterized in animals and bile was taken for 60 minutes, further
in order to assess the absorption and excretion function of the liver bromosulfalein test was performed. After that the animals were sacrificed by total bloodletting from the heart. Glycogen content was determined in the liver.
Results of the research and its discussion. Due to the simulation of combined abdominal and thoracic trauma during 1-7 days of the post-traumatic period liver disfunctional occurs in the body of the experimental animals, which is manifested by a slow release of bromosulfalein with bile and decrease of glycogen content in the liver. In conditions of isolated thoracic trauma, slowing of bromosulfalein release was observed after 1-3 days. After simulating isolated abdominal trauma and combined thoracoabdominal trauma bromosulfalein secretion was observed throughout the observation period with a maximum of 7 days in the combined trauma condition. The content of glycogen in the
liver after causing isolated thoracic trauma decreased after 1-3 days of post-traumatic period and until the 7th day returned to the level of the control group. In conditions of isolated abdominal trauma and combined thoracoabdominal trauma, glycogen synthesis liver function abnormality was larger and after 3 and 7 days of post-traumatic period exceeded animals with isolated thoracic trauma. Thiotriazoline under conditions of combined thoracoabdominal trauma by the level of absorption-excretory function is accompanied by an evident hepatoprotective effect, which indicates future outlook of the medication in the complex intensive therapy of traumatic disease in the acute period and in the period of its early manifestations, and requires further preclinical study.
Conclusions. In condition of combined abdominal and thoracic trauma in the acute period and the period of early manifestations of traumatic disease in the body of experimental animals absorption and excretion function as well as glycogen synthesis function of the liver are significantly impaired, which is manifested by a significant increase of bromosulfalein excretion with bile for 1-7 days with a maximum after causing of combined trauma, and a decrease of glycogen content in the liver after 3-7 days after
isolated trauma of the abdomen and combined trauma. The use of thiotriazoline is accompanied by a significant reduction in the duration of bromosulfalein excretion with bile and practically does not affect the glycogen content in the liver 

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Published
2019-09-11
How to Cite
GoodymaА., Zachepa, O., & Sushko, Y. (2019). THE INFLUENCE OF COMBINED ABDOMINAL AND THORACIC TRAUMA ON THE ABSORBINGEXCRETING AND GLYCOGEN-SYNTHESIZING FUNCTIONS OF THE LIVER IN THE PERIOD OF EARLY MANIFESTATIONS OF TRAUMATIC DISEASE AND THEIR CORRECTION WITH THIOTRIAZOLINE. Ukrainian Journal of Military Medicine, 19(3), 66-72. Retrieved from https://ujmm.org.ua/index.php/journal/article/view/56